The Mediterranean Journal of
Surgery and Medecine
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Diagnostic Role of
Immunogenetics in Post-Vaccine Diseases of the Central Nervous System (CNS): Preliminary Results
Associazione Universo Bambino - Italy
Montinari, Biagio Favoino, Angela
* Servizio di Tipizzazione tessutale e trapianti d'organo - Azienda Ospedaliera
Policlinico di Bari, Italy
** Ambulatorio di Virologia -
Azienda Ospedaliera Policlinico di Bari, Italy
We report of 30 patients with post-vaccine diseases of CNS and other organs who
presented with the initial symptoms at the time of or immediately a.fter vaccines.
All patients were tested for Herpes virtis (IgG and IgM) and tissue typing (HLA A,
B, C, HLA DR-DQ) to study the relationship between the development of post-vaccine CNS
diseases and those antigens trying to show that an immunogenetic substrate (autoimmune
type) is involved in demyeiination processes.
The comparison of our patients with formai Italian controis showed an increased
presence of HLA A3 and DR-7 antigens in the former group. The occurrence of A3 and/or DR7
was observed in 22/30 patients (73.3%)
Key words: Post-vaccine disease - HLA antigens
-Autoinimune disease CNS
Cette étude rapporte lobservation de 30 patients atteints de pathologies
post-vaccinales intéressant le SNC et dautres systèmes dans lesquels ies premiers
symptòmes sont apparus concomitamment ou immédiatement après ladministration de
Chez tous les patients on a effectué des recherches sérologiques pour les virus
herpétiques (IgG et IgM) ainsi quune caractérisation tissulaire HLA (A,B,C) et
HLA-DR-DQ afin de mettre en évidence tine corrélation possible entre lapparition
de pathologies citi SNC et ces antigènes; autoimmunitaires du SNC
cette corrélation pourrait expliquer une éventuelle base immunogénétique de
type autoimrnunitaire dans les processus de démyélinisation. La comparaison statistique
avec la population italienne de contròle a mis en évidence une augmentation des
antigènes HLA-A3 et HLA-DR-7. On a observé la présence de A3 et/ou DR-7 chez 22/30
Mots-clés: Pathologies post-vaccinales - Système HLA
Lo studio si riferisce allosservazione di 30 pazienti affetti da patologie
post-vaccinali con interessamento del SNC e di altri apparati nei quali i primi sintomi
sono insorti in concomitanza o immediatamente dopo la somministrazione di vaccini.
Tutti i pazienti sono stati sottoposti ad indagini sierologiche per virus erpetici
(IgG e IgM) nonché a tipizzazione tessutale HLA(A,B,C) e HLA-DR-DQ allo scopo di
accertare una eventuale correlazione tra linsorgenza di patologie a carico del SNC e
questi antigeni tale da spiegare una possibile base immunogenetica di tipo autoimmunitario
nei processi di demielinizzazione.
Il confronto statistico con la popolazione italiana di
controllo ha messo in evidenza un aumento degli antigeni HLA-A3 e HLADR-7.
di A3 e/o DR-7 è stata osservata in 22/30 (73,3%) dei pazienti.
Parole chiave: Patologie post-vaccinali - sistema HLA
- patologie autoimnuinitarie del SNC
CNS post-vaccine diseases are often not well studied.Recently,
it has been suggested the occurrence of CNS demyelination follwing immunisation with
recombinant Hepatitis B vaccine (1) ancl these observation have heen supported by
other Investigators who reported lack of virus and eosinophiiia following
recombinant Hepatitis B vaccine (2). These studies induced us to reevaiuate patients
who presented with netirological symptoms followingn immunisation already investigated in
several Itaiian and European Centres.
Med. J. Surg. Mcd. 2 (1996), 69-72
by Harris L. Coulter:
This is, to my knowledge, the first investigation to
find biochemical markers of vaccine damage.
It has not yet been published
but deserves publication. My translation omits the tables and part of the
bibliography, but the text is complete. This study should also have an
impact on HLA typing, since it shows that vaccinations can have an effect
on the individual's HLA type (i.e., that it is not necessarily congenital).
Italiana dello Studio
Immunogenetic (English study)
dei Vaccini + Falsità
della medicina ufficiale
Italian Study Finding Biochemical Markers of Vaccine Damage
(Ripubblicazione e copia negli USA di Harris L. Coulter Ph.D.
dello Studio del
dott. Massimo Montinari)
of Immunogenetics in the Diagnosis of Postvaccinal CNS Pathology -
Montinari*, Biagio Favoino**, and Angela Roberto*** Dept. Of Pediatric Surgery, University of Bari
**Tissue Typing and Organ Transplantation Service, Bari Hospital and
Polyclinic ***Virology Outpatient Clinic. Bari Hospital and Polyclinic
in Naples, May 9, 1996, under the auspices of the Associazione per la
Libera Universita Internazionale de Medicina Omeopatica "Samuel
Hahnemann" (LUIMO). Translated from Italian by
L. Coulter, Ph.D.
This study involves observations of 30 patients with post-vaccinal
pathology of the central nervous system and other systems where the first
symptoms appeared concomitantly with, or immediately after, administration
of a vaccine. All patients were subjected to serologic testing for herpes
virus (IgG and IgM) and to HLA (A, B, C) and HLA-DR-DQ tissue typing to
see if there was any correlation between the emergence of CNS pathology
and these various antigens, thus to show a possible autoimmune-type
immunogenetic basis for demyelination processes. Statistical comparison
with the Italian population used as controls revealed an increase in the
HLA-A3 and HLA-DR7 antigens. The presence of A3 and/or DR-7 was observed
in 22/30 (73.3%) of the patients.
Post-vaccinal pathology; HLA system; autoimmune pathology of the CNS.
Post-vaccinal pathology of the central nervous system (CNS) is a topic
deserving further investigation. In fact, our own experience with 30
patients of Italian nationality, observed between April, 1994 and October,
1995, shows that clinical signs of CNS pathology -- associated with
dermatitis, food allergies, constipation, and leaking from the anus --
emerged concomitantly or immediately after vaccination with the Salk or
Sabin polio vaccine, DT, measles, DPT, anti-tuberculosis, or Hepatitis-B
hypothesis of Herroelen, J. De Keyser, and G. Ebinger on "CNS
demyelination after immunization with recombinant hepatitis-B
vaccine" (Lancet, 338, November 9, 1991, 1174-1175), as verified by
A.P. Brezin, M. Lautier-Frau, M. Hamadani, and O. Rogeaux in their article,
"Loss of Vision and Eosinophilia after Recombinant Hepatitis-B
Vaccine" (Lancet, Italian Edition, April, 1994), suggests the need
for a clinical revaluation and a critical look at all the patients
observed up to now in Italian and European clinical centers.
The patients examined by us came from various regions of Italy, and all
presented with a clinical history of convulsions concomitantly with, or
immediately after, prophylactic vaccinations. We excluded from the study
all patients observed by us whose clinical history was not referable to a
vaccination. All the patients were subjected to tissue typing for HLA (A,
B, C) and HLA DR-DQ with the aim of defining the relative immunogenetic
order. The phenotype was defined by a study of various immune functions:
lymphocyte subpopulations, serum immunoglobulin content, sphericity of the
antibodies to various viruses (CMV, EBV, HSV-1 and HSV-2, VZV).
allowed us to relate these data to specific clinical pictures -- patients
who had earlier been diagnosed with epilepsy, myoclonic epilepsy, evoving
epilepsy, epileptigenic encephalopathy, autism, West Syndrome, and
All the patients had presented with the first
symptoms shortly after receiving the prophylactic vaccination or somewhat
first symptoms were convulsions, very high fever, or diarrhoea immediately
following a compulsory vaccination.
The parents had told their physicians
about this; then, after taking EEGs and visiting neuropsychiatric
specialists or pediatricians without getting any satisfaction, the
physicians had administered the recall shots of the vaccines leading very
shortly to stabilization of the condition with progressive clinical
children were mostly from 3 to 9 months old. All patients were studied for
the presence of metabolic diseases with negative results; then chromosomal
mapping was done, also with negative results; encephalic TAC and RMN were
performed at first appearance of the symptomatology, also with negative
EEG performed at first appearance of the symptomatology gave a negative
result in 92% of the patients. Serologic investigations for herpetic virus
(IgG and IgM) were positive in all for IgG and negative for all for IgM,
leading us to estimate seropositivity (IgG) for Epstein-Barr virus of
73.8%, for cytomegalovirus of 71.4%, for Herpes Simplex virus of 47.6%,
and for Varicella-Zoster Virus of 21.4%. In all the patients we observed
diminished sideremia and a deficit of IgA and IgG with a slight increase
of GOT and GPT.
None of the patients had maternally transmitted viral
encephalopathy, and in all the patients the vegetative and relational life
was quite normal prior to administration of the first dose of vaccine.
patients were subjected to HLA tissue typing (A, B, and C), and serologic
HLA DR-DQ, with the aim of checking a possible correlation with the
emergence of CNS pathology, and these antigens indicate a possible
autoimmune immunogenetic basis for the demyelination process. (See A.
Svejgard, P. Platz, and L. P. Ryder in Immunology Rev. 70, 1983, 193). The
chi-square statistical analysis, with the Italian population as a control
(see 11th International Histocompatibility Workship and Conference, 1992)
demonstrated an increase in the HLA-A3 antigen (43.3% vs. 25%, P = 0.04,
after statistical correction) and the HLA-DR7 antigen (48.3% vs. 24.14% P
= 0.007 after statistical correction). The presence of A3 and/or DR7 was
observed in 22/30 (73.3%) of the patients.
cases are under study to better define the possible association of HLA A3
and/or HLA DR7 with appearance of this pathology in the CNS following
vaccination. HLA system alleles have an elevated genetic polymorphism and
are inherited as autosomal dominant characteristics. The combination of
the alleles of various loci in the same chromosomes has been defined as
the haplotype or complex gene, and the complexity of the HLA region
demonstrates, besides the thousand different possible haplotypes, also the
problems: of molecular resemblance (see G. Laurentaci and B. Favoino,
"Immunogenetica e malattie HLA Associate," Dedalo Litostampo,
Bari, 1991), of discriminating between self- and non-self-antigens, and of
determining the function of the Class 2a CMI molecules; any interference
with the process of presentation of the antigen can predispose to an
autoimmune disease. Alterations which do not occur can be due to the
action of viral agents which compromise the specific immune response
because of their resemblance to the "self" tissue antigens. The
consequence is persistence of the infective agents and a tendency to
provoke, through a marked reaction, induction of an autoimmune disease.
This can present in conditions of marked reactivity to some viruses and to
study of the disease associated with genes of the HLA system has shown
that this genetic complex can be responsible for a particular genetic
susceptibility, predisposing to various diseases characterized
predominantly by immune-system pathogenesis.
The observation that many
vaccines use Thimerosal as a preservative, for which we do not have clear
dose-response relationships and whose toxic effects take the form
essentially of neurologic symptoms, not the least of which are symptoms of
the purine pathway of the innervation of the digestive tube, leads us to
consider that in 66% of cases there was obstinate constipation and in 31%
there was proctic symptomatology with emission of mucus and blood.
All the patients observed presented various physical problems. The various
types of CNS pathology could be due to a delatentization of preexisting
autoimme damage by viral DNA. It has been observed that the
“cleaner" the species, from the virologic or microbiologic point of
view, the more likely it is to present autoimmune conditions of the CNS
and other apparatuses. The results indicate that autoimmune pathology is
more frequent in countries where vaccination is more widespread, i.e., in
countries defined as "clean." With this study, and with the
individualization of alleles such as A3 and DR7, in the presence of viral
DNA, it would be possible to define the subjects at risk of an autoimmune
pathology from vaccination. The action of thimerosal used as an excipient
in vaccines, and whose toxicity is independent of thedose administered,
could demonstrate the possibility of changes in the aminoacids of the
molecules which preserve the antigen.
type of study could even be utilized to individualize the etiopathogenesis
of other types of autoimmune pathology.
An Italian Study Finding Biochemical Markers of Vaccine Damage
L. Coulter Ph.D.
che le alterazioni degli
pH digestivo e della
mucosa intestinale influenzano la
salute, non soltanto a livello intestinale, ma
anche a distanza in qualsiasi parte
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Interrogazione Parlamentare sui
danni dei Vaccini-1